Human Chromogranin A Monoclonal Antibody (CGA-2G8)

$295.00

In Stock

SKU: M2009 Categories: , Tag:
Quantity100µg
Price (USD)$295
ImmunogenFull length recombinant human CgA expressed in E. coli
ClonalityMonoclonal
CloneCgA-2G8
HostMouse IgG2b Clone CgA-2G8
ApplicationsELISA, WB, IP
TargetChromogranin A
ReactivityHuman
Conjugate/Tag/Label
Unconjugated
SwissProtIDP10645
EntrezGeneIDCHGA
FormulationLyophilized from PBS, trehalose
PurityProtein G purified
ImmunogenicityFull length recombinant human CgA expressed in E. coli
Product Storage2 years at -20°C to -80°C as supplied.
1 month at 4°C after reconstitution with preservative.
1 year at -20°C to -80°C after reconstitution.
PackagingLyophilized Vial
Shipment TypeAmbient
Shipping CarrierFedEx Overnight

Product Description

Chromogranin A (CgA), UniProt # P10645, is a 439 amino acid member of the granin family of secretory proteins. Found in vesicles of neuroendocrine cells, CgA is considered a valuable marker for neuroendocrine tumors. CgA is a precursor to a number of biologically active peptides including vasostatin I, parastatin, and pancreastatin.

Sourced from mouse monoclonal IgG1 with reactivity in humans. Targets CgA and is a clone of CGA-2G8. Protein G purified from hybridoma cell culture supernatant. Applications include ELISA, Western Blot Analysis, and Immunoprecipitation. Arrives in a Lyophilized Vial shipped by FedEx Overnight.

For Research Use Only.


Storage & Reconstitution

  • Lyophilized from PBS with Trehalose to keep for 2 years as supplied.
  • Reconstitute in 100μL ddH20 to 1 mg/mL, store for 1 month at 4°C after reconstitution with preservative.
  • For long-term storage, store for up to 1 year at -20°C to -80°C after reconstitution.
  • Expires 2 years from the date of receipt.

Human CgA Luminex Assay

  • Capture: Human CgA monoclonal clone CgA-3H11 (Cat # M2007).
  • Detection: Human CgA monoclonal clone CgA-2G8 (Cat # M2009).
  • Antigen: Recombinant human CgA, E. coli expressed (Cat # P4006).

Immunoprecipitation of Native CgA

SDS PAGE 4-12%, reducing conditions.

Blocking: 5% NFDM, PBS.

Sample: Human CgA purified from human serum with M2009.

Blot was probed with 4µg/mL M2009 followed by HRP detection antibody. Human CgA was detected at 52kDa.

Applications

Immunoprecipitation, Western Blot, ELISA Testing

Reactivity