$295.00

SKU: R3005 Categories: , Tag:
Quantity100µg
Price (USD)$295
ImmunogenFull length recombinant human Gelsolin expressed in E. coli
ClonalityPolyclonal
ClonePolyclonal
HostRabbit Polyclonal
ApplicationsELISA, WB, IP
TargetGelsolin
ReactivityHuman
Conjugate/Tag/Label
Unconjugated
SwissProtIDP06396
EntrezGeneIDGSN
Accession NumberNP_000168
FormulationAntigen affinity purified
PurityProtein G purified
Product Storage2 years at -20°C to -80°C as supplied.
1 month at 4°C after reconstitution with preservative.
1 year at -20°C to -80°C after reconstitution.
PackagingLyophilized Vial
Shipment TypeAmbient
Shipping CarrierFedEx Overnight

Product Description

Human Gelsolin (UnitProt # P06396) is a potent calcium regulated actin filament severing protein that works to disassemble and reorganize the actin network. The extracellular isoform, plasma gelsolin, is suggested to act as an actin scavenging protein. Decreased levels of plasma gelsolin have been reported in trauma, burns, rheumatoid arthritis, and Alzheimer disease.

Sourced from rabbit polyclonal IgG with reactivity in humans, bovine, swine, horses, goats, canines, and guinea pigs. Targets Gelsolin. Antigen affinity purified. Applications include ELISA testing, Western Blot analysis, and Immunoprecipitation. Arrives in a Lyophilized Vial shipped by FedEx Overnight.

For Research Use Only.


Storage & Reconstitution

  • Lyophilized from PBS with Trehalose to keep for 2 years as supplied.
  • Reconstitute in 100μL ddH20 to 1 mg/mL, store for 1 month at 4°C after reconstitution with preservative.
  • For long-term storage, store for up to 1 year at -20°C to -80°C after reconstitution.
  • Expires 2 years from the date of receipt.

Western Blot Detection of Human Gelsolin

SDS PAGE 4-12%, reducing conditions.

Blocking: 5% NFDM, PBS.

  • Lane 1: Recombinant human gelsolin (Cat # P4004), 10ng.
  • Lane 2: Albumin depleted human plasma, 100µg.
  • Lane 3: Albumin depleted human plasma, 10µg.

Blot was probed with 0.2µg/mL R3005 followed by HRP detection antibody. Human gelsolin was detected at 95kDa.

Applications

Immunoprecipitation, Western Blot, ELISA Testing

Reactivity