Human Chromogranin B Monoclonal Antibody (CgB-1E11)

$295.00

In Stock

SKU: M2015 Categories: , Tag:
Quantity100µg
Price (USD)$295
ImmunogenFull length recombinant human CgB expressed in HEK293
ClonalityMonoclonal
CloneCgB-1E11
HostMouse IgG1 Clone CgB-1E11
ApplicationsELISA, WB
TargetCgB
ReactivityHuman
Conjugate/Tag/Label
Unconjugated
SwissProtIDP05060
EntrezGeneIDCHGB
FormulationLyophilized from PBS, trehalose
PurityProtein G purified
ImmunogenicityFull length recombinant human CgB expressed in HEK293
Product Storage2 years at -20°C to -80°C as supplied.
1 month at 4°C after reconstitution with preservative.
1 year at -20°C to -80°C after reconstitution.
PackagingLyophilized Vial
Shipment TypeAmbient
Shipping CarrierFedEx Overnight

Product Description

Chromogranin B (CgB) aka Secretogranin-1, UniProt # P05060, is an acidic member of the granin family found in a variety of neurons and endocrine cells. CgB is a marker in neuroendocrine tumors with plasma levels potentially being associated with disease progression.

Sourced from mouse monoclonal IgG1 with reactivity in humans. Targets Chromogranin and is a clone of CgB-1E11. Protein G purified from hybridoma cell culture supernatant. Applications include ELISA and Western Blot Analysis. Arrives in a Lyophilized Vial shipped by FedEx Overnight.

For Research Use Only.


Storage & Reconstitution

  • Lyophilized from PBS with Trehalose to keep for 2 years as supplied.
  • Reconstitute in 100μL ddH20 to 1 mg/mL, store for 1 month at 4°C after reconstitution with preservative.
  • For long-term storage, store for up to 1 year at -20°C to -80°C after reconstitution.
  • Expires 2 years from the date of receipt.

Human CgB Luminex Assay

  • Capture: Human CgB monoclonal clone CgB-1E11 (Cat # M2015).
  • Detection: Human CgB antigen purified polyclonal.
  • Antigen: Recombinant human CgB (Cat # P4007).

Western Blot Detection of Native CgB

SDS PAGE 4-12%, reducing conditions.

Blocking: 5% NFDM, PBS.

Sample: HEK293 whole cell lysate, 76µg.

Blot was probed with 4µg/mL M2015 followed by HRP detection antibody. Human CgB was detected at 76kDa.

Applications

Western Blot, ELISA Testing

Reactivity