Human Vaspin Monoclonal Antibody (VPN-3C4)

$295.00

SKU: M2016 Categories: , Tag:
Quantity100µg
Price (USD)$295
ImmunogenFull length recombinant human Vaspin expressed in HEK293
ClonalityMonoclonal
CloneVPN-3C4
HostMouse IgG1 Clone VPN-3C4
ApplicationsELISA, WB, IP
TargetVaspin
ReactivityHuman
Conjugate/Tag/Label
Unconjugated
SwissProtIDQ8IW75
EntrezGeneIDSERPINA12
Accession NumberNP_776249
FormulationLyophilized from PBS, trehalose
PurityProtein G purified
ImmunogenicityFull length recombinant human Vaspin expressed in HEK293
Product Storage2 years at -20°C to -80°C as supplied.
1 month at 4°C after reconstitution with preservative.
1 year at -20°C to -80°C after reconstitution.
PackagingLyophilized Vial
Shipment TypeAmbient
Shipping CarrierFedEx Overnight

Product Description

Visceral adipose tissue-derived serpin (Vaspin) aka SerpinA12, UniProt # Q8IW75, is an adipokine originally isolated from visceral adipose tissue and is a member of the Serpin family of serine protease inhibitors. Increased levels of vaspin are observed in obesity as well as type 2 diabetes and its expression positively correlates to body mass index and insulin sensitivity.

Sourced from mouse monoclonal IgG1 with reactivity in humans. Targets Vaspin and is a clone of VPN-3C4. Protein G purified from hybridoma cell culture supernatant. Applications include ELISA, Immunoprecipitation, and Western Blot Analysis. Arrives in a Lyophilized Vial shipped by FedEx Overnight.

For Research Use Only.


Storage & Reconstitution

  • Lyophilized from PBS with Trehalose to keep for 2 years as supplied.
  • Reconstitute in 100μL ddH20 to 1 mg/mL, store for 1 month at 4°C after reconstitution with preservative.
  • For long-term storage, store for up to 1 year at -20°C to -80°C after reconstitution.
  • Expires 2 years from the date of receipt.

Human Vaspin Luminex Assay

  • Capture: Human Vaspin monoclonal clone VPN-3C4 (Cat # M2016).
  • Detection: Human Vaspin antigen purified polyclonal (Cat # R3000).
  • Antigen: Recombinant Human Vaspin (Cat # P4004).

Immunoprecipitation of Native Vaspin

SDS PAGE 4-12%, reducing conditions.

Blocking: 5% NFDM, PBS.

Sample: Human Vaspin purified from human plasma with M2016.

Blot was probed with 4µg/mL M2016 followed by HRP detection antibody. Human Vaspin was detected at 52kDa.

Antigen: Recombinant Human Vaspin (Cat # P4004).

Applications

Immunoprecipitation, Western Blot, ELISA Testing

Reactivity