Human Vaspin Polyclonal Antibody

$295.00

SKU: R3000 Categories: , Tag:
Quantity100µg
Price (USD)$295
ImmunogenFull length recombinant human Vaspin expressed in HEK293
ClonalityPolyclonal
ClonePolyclonal
HostRabbit Polyclonal
ApplicationsELISA, WB
TargetVaspin
ReactivityHuman
Conjugate/Tag/Label
Unconjugated
SwissProtIDQ8IW75
EntrezGeneIDSERPINA12
Accession NumberNP_776249
FormulationAntigen affinity purified
PurityProtein G purified
Product Storage2 years at -20°C to -80°C as supplied.
1 month at 4°C after reconstitution with preservative.
1 year at -20°C to -80°C after reconstitution.
PackagingLyophilized Vial
Shipment TypeAmbient
Shipping CarrierFedEx Overnight

Product Description

Visceral adipose tissue-derived serpin (Vaspin) aka SerpinA12, UniProt # Q8IW75, is an adipokine originally isolated from visceral adipose tissue and is a member of the Serpin family of serine protease inhibitors. Increased levels of vaspin are observed in obesity as well as type 2 diabetes and its expression positively correlates to body mass index and insulin sensitivity.

Sourced from rabbit polyclonal IgG with reactivity in humans derived from H293. Targets Vaspin. Antigen affinity purified. Applications include ELISA testing and Western Blot analysis. Arrives in a Lyophilized Vial shipped by FedEx Overnight.

For Research Use Only.


Storage & Reconstitution

  • Lyophilized from PBS with Trehalose to keep for 2 years as supplied.
  • Reconstitute in 100μL ddH20 to 1 mg/mL, store for 1 month at 4°C after reconstitution with preservative.
  • For long-term storage, store for up to 1 year at -20°C to -80°C after reconstitution.
  • Expires 2 years from the date of receipt.

Western Blot Detection of Native Vaspin

SDS PAGE 4-12%, reducing conditions.

Blocking: 5% NFDM, PBS.

Sample: 70µg albumin depleted human plasma.

Blot was probed with 1µg/mL R3000 followed by HRP detection antibody.

Human Vaspin was detected at 51kDa.

Applications

Western Blot, ELISA Testing

Reactivity