Ferret CD56 Monoclonal Antibody (FT56-4B3)

$295.00

SKU: M2039 Categories: , Tag:
Quantity100µg
Price (USD)$295
ImmunogenExtracellular domain recombinant ferret CD56 expressed in HEK293 cells
ClonalityMonoclonal
CloneFT56-4B3
HostMouse IgG1 Clone FT56-4B3
ApplicationsFlow Cytometry
TargetCD56
ReactivityFerret
Conjugate/Tag/Label
Unconjugated
SwissProtIDA0A8U0RZ03
EntrezGeneIDNCAM1
Accession NumberXP_044933500
FormulationLyophilized from PBS, trehalose
PurityProtein G purified
Product Storage2 years at -20°C to -80°C as supplied.
1 month at 4°C after reconstitution with preservative.
1 year at -20°C to -80°C after reconstitution.
PackagingLyophilized Vial
Shipment TypeAmbient
Shipping CarrierFedEx Overnight

Product Description

CD56, UniProt # A0A8U0RZ03, also known as NCAM, is a glycoprotein of the Ig superfamily. It serves as a marker for natural killer cells.

Sourced from mouse monoclonal IgG1 with reactivity in ferrets. Targets CD56 and is a clone of FT56-4B3. Protein G purified from hybridoma cell culture supernatant. Applications include flow cytometry. Arrives in a Lyophilized Vial shipped by FedEx Overnight.

For Research Use Only.


Storage & Reconstitution

  • Lyophilized from PBS with Trehalose to keep for 2 years as supplied.
  • Reconstitute in 100μL ddH20 to 1 mg/mL, store for 1 month at 4°C after reconstitution with preservative.
  • For long-term storage, store for up to 1 year at -20°C to -80°C after reconstitution.
  • Expires 2 years from the date of receipt.

Flow Cytometric Analysis of CD56 Expression on Ferret PBMCs

Representative double staining for female ferret PBMCs stained with biotinylated mouse anti-ferret antibodies followed by streptavidin PE) in conjunction with ferret cross-reactive antibodies described in the literature. Cells were first gated on a live/dead stain and doublets removed before gating on forward and side scatter. Shown is the total lymphocyte population double staining with anti-mouse CD11b and each mouse biotinylated antibody (Fig A-E). CD64 (Cat No. M2038) and CD86 (Cat No. M2040) co-stain with macrophage marker CD11b as expected, while the NK marker CD56 does not co-stain with CD11b. In panels F-H, cells were double stained with human anti-CD79, a B cell marker. CD64 and CD86 do costain to some extent with the B cell marker as B cells are known to express FcgRI and the co-stimulatory molecule CD86. A small percentage of CD56+ cells also stain positive for CD79 marker.

Applications

Flow Cytometry

Reactivity