Ferret CD86 Monoclonal Antibody (FT86-1H1)

CD86, UniProt # A0A8U0MKT0, is a type I membrane protein and a member of the Ig superfamily of proteins. It is expressed on dendritic cells, macrophages, B cells, and other antigen presenting cells.

Applications	Flow Cytometry
Reactivity	Ferret

$295.00

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Product Description

Sourced from mouse monoclonal IgG1 with reactivity in ferrets. Targets CD86 and is a clone of FT86-1H1. Protein G purified from hybridoma cell culture supernatant. Applications include flow cytometry. Arrives in a Lyophilized Vial shipped by FedEx Overnight.

For Research Use Only.

Storage & Reconstitution

Flow Cytometry

Flow Cytometric Analysis of CD86 Expression on Ferret PBMCs

Representative double staining for female ferret PBMCs stained with biotinylated mouse anti-ferret antibodies followed by streptavidin PE) in conjunction with ferret cross-reactive antibodies described in the literature. Cells were first gated on a live/dead stain and doublets removed before gating on forward and side scatter. Shown is the total lymphocyte population double staining with anti-mouse CD11b and each mouse biotinylated antibody (Fig A-E). CD64 (Cat No. M2038) and CD86 clone 1H1 co-stain with macrophage marker CD11b as expected, while NK marker CD56 (Cat No. M2039) does not co-stain with CD11b. In panels F-H, cells were double stained with human anti-CD79, a B cell marker. CD64 and CD86 do co-stain to some extent with the B cell marker as B cells are known to express FcgRI and the costimulatory molecule CD86. A small percentage of CD56+ cells also stain positive for CD79 marker.

Product Details

Quantity	100µg
Price (USD)	$295
Immunogen	Extracellular domain recombinant ferret CD86 expressed in HEK293 cells
Clonality	Monoclonal
Clone	FT86-1H1
Host	Mouse IgG₁ Clone FT86-1H1
Applications	Flow Cytometry
Target	CD86
Reactivity	Ferret
Conjugate/Tag/Label	Unconjugated
SwissProtID	A0A8U0MKT0
EntrezGeneID	CD86
Accession Number	XP_004745529
Formulation	Lyophilized from PBS, trehalose
Purity	Protein G purified
Product Storage	2 years at -20°C to -80°C as supplied. 1 month at 4°C after reconstitution with preservative. 1 year at -20°C to -80°C after reconstitution.
Packaging	Lyophilized Vial
Shipment Type	Ambient
Shipping Carrier	FedEx Overnight