Hamster CD69 Monoclonal Antibody (HM69-2B3) Biotin
$375.00
| Quantity | 100µg |
| Immunogen | Recombinant hamster CD69 Ala83-Arg206 w/ C-terminal His tag expressed in HEK293 |
| Clonality | Monoclonal |
| Clone | HM69-2B3 |
| Host | Mouse IgG1 |
| Applications | Flow Cytometry |
| Target | CD69 |
| Reactivity | Hamster |
| Conjugate/Tag/Label | Biotin |
| Formulation | Lyophilized from PBS, trehalose |
| Purity | Protein G purified |
| Product Storage | 2 years at -20°C to -80°C as supplied. 1 month at 4°C after reconstitution with preservative. 1 year at -20°C to -80°C after reconstitution. |
| Packaging | Lyophilized Vial |
| Shipment Type | Ambient |
| Shipping Carrier | FedEx Overnight |
Mouse monoclonal IgG1 reactive with hamster CD69. M2074 (clone HM69-2B3) has been used in flow cytometry to detect hamster CD69. Protein G purified from hybridoma cell culture supernatant. Arrives lyophilized by FedEx overnight.
For Research Use Only.
Hamster CD69 Flow Cytometry
Anti-CD25 clone HM25-3G4 binding increased on CD4 cells after 24 h and 48 h activation with Con A.
Anti-CD69 clone HM69-2B3 showed increased levels after 24 h, but less expression after 48 h which
was expected with CD69 being an early activation marker. Hamster spleen cells were incubated with
Concanavalin A for 24 (A) or 48 (B) hours. For all samples, dead cells were first gated out based on a
live-dead dye and live cells further gated on CD4+ cells. CD4+ cells were then analyzed for CD69 and
CD25 expression.
| Applications | Flow Cytometry |
|---|---|
| Reactivity |