Hamster CD69 Monoclonal Antibody (HM69-2B3) Biotin

$375.00

SKU: M2074b Category: Tag:
Quantity100µg
ImmunogenRecombinant hamster CD69 Ala83-Arg206 w/ C-terminal His tag expressed
in HEK293
ClonalityMonoclonal
CloneHM69-2B3
HostMouse IgG1
ApplicationsFlow Cytometry
TargetCD69
ReactivityHamster
Conjugate/Tag/Label
Biotin
FormulationLyophilized from PBS, trehalose
PurityProtein G purified
Product Storage2 years at -20°C to -80°C as supplied.
1 month at 4°C after reconstitution with preservative.
1 year at -20°C to -80°C after reconstitution.
PackagingLyophilized Vial
Shipment TypeAmbient
Shipping CarrierFedEx Overnight

Product Description

Mouse monoclonal IgG1 reactive with hamster CD69.  M2074 (clone HM69-2B3) has been used in flow cytometry to detect hamster CD69.  Protein G purified from hybridoma cell culture supernatant.   Arrives lyophilized by FedEx overnight.

For Research Use Only.


Hamster CD69 Flow Cytometry

Anti-CD25 clone HM25-3G4 binding increased on CD4 cells after 24 h and 48 h activation with Con A.
Anti-CD69 clone HM69-2B3 showed increased levels after 24 h, but less expression after 48 h which
was expected with CD69 being an early activation marker. Hamster spleen cells were incubated with
Concanavalin A for 24 (A) or 48 (B) hours. For all samples, dead cells were first gated out based on a
live-dead dye and live cells further gated on CD4+ cells. CD4+ cells were then analyzed for CD69 and
CD25 expression.

Applications

Flow Cytometry

Reactivity