Ferret CD64 Monoclonal Antibody (FT64-3E6)
$295.00
| Quantity | 100µg |
| Price (USD) | $295 |
| Immunogen | Extracellular domain recombinant ferret CD64 expressed in HEK293 cells |
| Clonality | Monoclonal |
| Clone | FT64-3E6 |
| Host | Mouse IgG1 Clone FT64-3E6 |
| Applications | Flow Cytometry |
| Target | CD64 |
| Reactivity | Ferret |
| Conjugate/Tag/Label | Unconjugated |
| SwissProtID | A0A8U0SCD3 |
| EntrezGeneID | FCGR1A |
| Accession Number | XP_044939077 |
| Formulation | XP_044939077 |
| Purity | Protein G purified |
| Product Storage | 2 years at -20°C to -80°C as supplied. 1 month at 4°C after reconstitution with preservative. 1 year at -20°C to -80°C after reconstitution. |
| Packaging | Lyophilized Vial |
| Shipment Type | Ambient |
| Shipping Carrier | FedEx Overnight |
Product Description
CD64, UniProt # A0A8U0SCD3, is an Fc-gamma receptor 1 (FcRI) that binds monomeric IgG with high affinity. It is expressed on monocytes and macrophages.
Sourced from mouse monoclonal IgG1 with reactivity in ferrets. Targets CD64 and is a clone of FT64-3E6. Protein G purified from hybridoma cell culture supernatant. Applications include flow cytometry. Arrives in a Lyophilized Vial shipped by FedEx Overnight.
For Research Use Only.
Storage & Reconstitution
- Lyophilized from PBS with Trehalose to keep for 2 years as supplied.
- Reconstitute in 100μL ddH20 to 1 mg/mL, store for 1 month at 4°C after reconstitution with preservative.
- For long-term storage, store for up to 1 year at -20°C to -80°C after reconstitution.
- Expires 2 years from the date of receipt.
Flow Cytometric Analysis of CD64 Expression on Ferret PBMCs
One million PBMCs were stained with biotinylated mouse anti-ferret CD64 clone 3E6, (followed by streptavidin APC-Cy7) in conjunction with ferret cross-reactive antibodies described in the literature. Non-specific binding was blocked using mouse FcR antibody to CD32. Ferret PBMCs stained with mouse anti-ferret CD64-3E6 also express CD11b and CD14, but not CD4 or CD8. The total population shown was further gated on CD64 negative (top panels) or CD64 positive cells (bottom panels). Histograms show expression of CD4, CD8, CD11b, CD14 and CD79b in CD64 negative (top panels) or CD64 positive (bottom panels) cells. CD64 negative cells express CD4, CD8 and CD79b, (lymphocyte markers) while CD64 positive cells express CD11b and CD14, (macrophage markers). CD64 positive cells also express CD79b and could represent B cells that express FcR.
| Applications | Flow Cytometry |
|---|---|
| Reactivity |
