Ferret CD86 Monoclonal Antibody (FT86-1H1), biotin
$350.00
| Quantity | 100µg |
| Price (USD) | 350 |
| Immunogen | Extracellular domain recombinant ferret CD86 expressed in HEK293 cells |
| Clonality | Monoclonal |
| Clone | FT86-1H1 |
| Host | Mouse IgG1 Clone FT86-1H1 |
| Applications | Flow Cytometry |
| Target | CD86 |
| Reactivity | Ferret |
| Conjugate/Tag/Label | Biotin |
| SwissProtID | A0A8U0MKT0 |
| EntrezGeneID | CD86 |
| Accession Number | XP_004745529 |
| Formulation | Lyophilized from PBS, trehalose |
| Purity | Protein G purified |
| Product Storage | 2 years at -20°C to -80°C as supplied. 1 month at 4°C after reconstitution with preservative. 1 year at -20°C to -80°C after reconstitution. |
| Packaging | Lyophilized Vial |
| Shipment Type | Ambient |
| Shipping Carrier | FedEx Overnight |
Product Description
CD86, UniProt # A0A8U0MKT0, is a type I membrane protein and a member of the Ig superfamily of proteins. It is expressed on dendritic cells, macrophages, B cells, and other antigen presenting cells.
Sourced from mouse monoclonal IgG1 coupled to biotin with reactivity in ferrets. Targets CD86 and is a clone of FT86-1H1. Protein G purified from hybridoma cell culture supernatant. Applications include flow cytometry. Arrives in a Lyophilized Vial shipped by FedEx Overnight.
For Research Use Only.
Storage & Reconstitution
- Lyophilized from PBS with Trehalose to keep for 2 years as supplied.
- Reconstitute in 100μL ddH20 to 1 mg/mL, store for 1 month at 4°C after reconstitution with preservative.
- For long-term storage, store for up to 1 year at -20°C to -80°C after reconstitution.
- Expires 2 years from the date of receipt.
Flow Cytometric Analysis of CD86 Expression on Ferret PBMCs
Representative double staining for female ferret PBMCs stained with biotinylated mouse anti-ferret antibodies followed by streptavidin PE) in conjunction with ferret cross-reactive antibodies described in the literature. Cells were first gated on a live/dead stain and doublets removed before gating on forward and side scatter. Shown is the total lymphocyte population double staining with anti-mouse CD11b and each mouse biotinylated antibody (Fig A-E). CD64 (Cat No. M2038) and CD86 clone 1H1 co-stain with macrophage marker CD11b as expected, while NK marker CD56 (Cat No. M2039) does not co-stain with CD11b. In panels F-H, cells were double stained with human anti-CD79, a B cell marker. CD64 and CD86 do co-stain to some extent with the B cell marker as B cells are known to express FcgRI and the co-stimulatory molecule CD86. A small percentage of CD56+ cells also stain positive for CD79 marker.
| Applications | Flow Cytometry |
|---|---|
| Reactivity |
